Hi - I am trying to dilute primers for a qPCR reaction. I'm a bit lost. Here is an example of one of my primers:

Came as 97.7 nmol to which I added 977 ul ... I believe this gives me a stock solution of 100 uM/L

I need to figure out how much of this solution I should use so that I am adding 0.5ng of primer to each reaction.

Any help would be much appreciated!

Came as 97.7 nmol to which I added 977 ul ... I believe this gives me a stock solution of 100 uM/L

I need to figure out how much of this solution I should use so that I am adding 0.5ng of primer to each reaction.

Any help would be much appreciated!

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