If I make a calibration curve with HPLC. On the X ax is mg/l and on the Y-ax is the area.

I dilute for example 0,5 gram solid or liquid in a 10 ml volumetric flask and make it up to volume with eluent. After some sample prep I inject the solution into the HPLC and found a area which has a concentration of 4 mg/l compared to the calibration curve. If we suggest that the slope goes through zero it makes a concentration of area unknown/slope.

After this I want to calculate the concentration in mg/kg. Am I right if I do it in this way.

Area/slope = concentration 4 mg/l absolute

But the sample weighted was in 10 ml volumetric flask so 1000 ml/10 = 100

4mg/l / 100 = 0.04 mg in 0,5 gram

1000 gram / 0.5 gram = 2000

2000*0.5 = 1000 mg/kg

Is this the right way to do it?

I dilute for example 0,5 gram solid or liquid in a 10 ml volumetric flask and make it up to volume with eluent. After some sample prep I inject the solution into the HPLC and found a area which has a concentration of 4 mg/l compared to the calibration curve. If we suggest that the slope goes through zero it makes a concentration of area unknown/slope.

After this I want to calculate the concentration in mg/kg. Am I right if I do it in this way.

Area/slope = concentration 4 mg/l absolute

But the sample weighted was in 10 ml volumetric flask so 1000 ml/10 = 100

4mg/l / 100 = 0.04 mg in 0,5 gram

1000 gram / 0.5 gram = 2000

2000*0.5 = 1000 mg/kg

Is this the right way to do it?

## Comment