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calculate nM to nM/gram

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  • calculate nM to nM/gram

    please help. i've determined the concentration of a solution to be 4.3nM
    This was done my taking 0.5ml of (1mg/mL) solution and combining to 2ml of another solution. so total volume was 2.5ml.

    after calculations, i get 4.3mM for that solution. how do i convert this to nM/gram of stuff? MW is 278.

    thanks

  • #2
    Re: calculate nM to nM/gram

    Originally posted by ptmagical View Post
    please help. i've determined the concentration of a solution to be 4.3nM
    This was done my taking 0.5ml of (1mg/mL) solution and combining to 2ml of another solution. so total volume was 2.5ml.

    after calculations, i get 4.3mM for that solution. how do i convert this to nM/gram of stuff? MW is 278.

    thanks
    nM is a concentration, so am assuming you want nanomoles per gram.

    You started with 0.5mg of your material in 2.5mL.
    Resulting concentration is 4.3mM, or 4.3 millimoles/litre. (There are 1000mL in 1L)


    You have 2.5/1000 x 4.3 mmoles = 0.01075mmoles = 10.75nmoles in 0.5mg.
    There are 1000mg in one gram, so 1000/0.5 x 10.75 = 21500nmoles per gram.

    ps, i've noticed you have put 4.3nM in one place and 4.3mM in another place. 4.3mM (4.3 millimoles per litre) is 1000 times more concentrated than 4.3nM (4.3 nanomoles per litre). If you want a check, please can you post back with the correct units?
    Last edited by Mrs X; 06-05-2013, 01:49 PM.

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    • #3
      Re: calculate nM to nM/gram

      my resulting amount is nanomoles

      so 4.3 nanomoles

      how do i convert this back to see it as nanomoles per gram?

      i weighed out 50mg into 50ml v.f. resulting in a 1mg/ml solution. from this, i took .5ml and added it to 2mls, so final volume was 2.5ml.

      i hope this makes sense

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      • #4
        Re: calculate nM to nM/gram

        Originally posted by ptmagical View Post
        my resulting amount is nanomoles

        so 4.3 nanomoles

        how do i convert this back to see it as nanomoles per gram?

        i weighed out 50mg into 50ml v.f. resulting in a 1mg/ml solution. from this, i took .5ml and added it to 2mls, so final volume was 2.5ml.

        i hope this makes sense
        Hi, let me know if this doesn't make sense:

        There are 1000mg in 1 gram

        You took 0.5mL of your 1mg/mL solution. Therefore you have 0.5mg of your material. You dilute it to 2.5mL.

        Assuming you test the whole 2.5mL, you have 4.3 nanomoles of your compound of interest in 0.5mg of your material. This is the same as 8.6 nanomoles in 1mg, which is equal to 8600 nanomoles in 1 gram.

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        • #5
          Re: calculate nM to nM/gram

          Mrs.X
          Thank you very much!! it all makes sense now, and your explanation was crystal clear. thank you again.

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          • #6
            Re: calculate nM to nM/gram

            You are more than welcome, good luck with your studies.

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