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  • calculate IU

    Hi guys,

    I have a solution to prepare but for some reason i am stuck..
    I have a vial (1.5ml) with 300 IU/ml FSH.. From that I need to use just a 10 IU/ml. So I need to take from the vial just 50 microliters? this equals 10 IU/ ml, right?
    In addition, I need to make a solution with 10 IU/ml FSH into PBS, although I do not know the final volume that the FSH/PBS must have.. Although I need just 1ml of the final solution

    Any help mych appreciated..
    Gio

  • #2
    Re: calculate IU

    Originally posted by gio_1980
    Hi guys,

    I have a solution to prepare but for some reason i am stuck..
    I have a vial (1.5ml) with 300 IU/ml FSH.. From that I need to use just a 10 IU/ml. So I need to take from the vial just 50 microliters? this equals 10 IU/ ml, right?
    In addition, I need to make a solution with 10 IU/ml FSH into PBS, although I do not know the final volume that the FSH/PBS must have.. Although I need just 1ml of the final solution

    Any help mych appreciated..
    Gio
    If you need 1 mL of 10 IU/mL solution, then you need 10 IU.

    You must withdraw 10 IU from a stronger solution of 300 IU/mL, hence you need
    10 IU x 1 mL/300 IU = 33.3 L of the stronger solution.
    That must be diluted to a final volume of 1 mL with water, or whatever the appropriate liquid is.

    You might make an amount that avoids messy fractions after the division
    0.9 mL: Use 30 L, dilute to 0.9 mL
    1.2 mL: Use 40 L, dilute to 1.2 mL

    Actually, use x, dilute to final volume of 30x
    Last edited by JohnS; 03-02-2009, 12:16 PM.

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    • #3
      Re: calculate IU

      Thank you very much John

      Comment


      • #4
        Re: calculate IU

        In addition, you would normally make these final solutions up in a volumetric flask, as you can get small volume changes whn mixing different solutions.

        Comment


        • #5
          How to calculate the enzyme units

          hi guys,
          after induction i purified the sample and i got the required protein by protein purification technique. i got the purified protein in three elutes. elute-1, elute-2, elute-3. by lowry method i got the value of 0.032mg/ml. i used 5 mL of bacterial culture.
          I used 150microlitres to elute the sample for three times.
          May i know how to calculate IU/mL.

          Comment


          • #6
            Re: calculate IU

            You have 300 IU/cc. You need 10 IU.

            It is unusual to have a vial label which does not have the concentration as amount per cc verses amount concentrated as you stated.

            For a volume this small, I would draw up 0.33 cc (100 IU) and dilute into a total volume of 10 cc, resulting in a concentration of 10 IU/cc.

            Another way would be to draw up 1.0 cc and dilute to a total volume of 30 cc, resulting in a concentration of 10 IU/cc.

            Another way would be to draw up 0.5 cc and dilute to a total volume of 15 cc, resulting in a concentration of 10 IU/cc.

            Regards
            C
            Last edited by Carl M; 02-28-2011, 04:37 PM.

            Comment


            • #7
              Re: How to calculate the enzyme units

              Originally posted by Unregistered View Post
              hi guys,
              after induction i purified the sample and i got the required protein by protein purification technique. i got the purified protein in three elutes. elute-1, elute-2, elute-3. by lowry method i got the value of 0.032mg/ml. i used 5 mL of bacterial culture.
              I used 150microlitres to elute the sample for three times.
              May i know how to calculate IU/mL.
              IU is a measure of activity, not mass, volume or weight. It is completely dependent on the item being measured.

              Comment


              • #8
                calculate IU

                I have TSH 10 UG stock. I need a 100 IU/mL TSH solution. Any Idea how to make this solution.

                Arjmand

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                • #9
                  Re: calculate IU

                  What is the volume of the UG? Also, exactly what is UG? IU is international units.

                  Comment


                  • #10
                    Re: convertion of IU to milligrams

                    I have a vial consisting of 100mu/vl of penicillin G. I have to prepare a solution with a concentration of 10micrograms/ml. I am stuck at this point. Any help in this regard will be appreciated

                    Cheers
                    Vasu

                    Comment

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