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increase dna concentration and convert from ng/uL to ug/uL

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  • increase dna concentration and convert from ng/uL to ug/uL

    Hello,
    I have a DNA sample that is 660.92 ng/uL and I need the total amount of DNA per reaction to be 5 ug. The total volume of the reaction is 25uL. I believe I can just increase the amount of DNA that I use but don't know the right calculation. Would appreciate any help.
    Kelly

  • #2
    Re: increase dna concentration and convert from ng/uL to ug/uL

    Originally posted by kellekat View Post
    Hello,
    I have a DNA sample that is 660.92 ng/uL and I need the total amount of DNA per reaction to be 5 ug. The total volume of the reaction is 25uL. I believe I can just increase the amount of DNA that I use but don't know the right calculation. Would appreciate any help.
    Kelly
    Well 5 g = 5000 ng, so
    5000 ng x 1 L/660.92 ng = 7.565 L.

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