Announcement

Collapse
No announcement yet.

Help with reactivation calculations

Collapse
X
  • Filter
  • Time
  • Show
Clear All
new posts

  • Help with reactivation calculations

    Hi everyone.

    I am looking to reactivate my CD4 t cells with a final concentration 50 ng/ml PMA plus 1μM ionomycin. I currently have both in 20uL aliquots at a concentration of 1 mg/mL. My cells are in 1mL media.

    I need help figuring out how to dilute the PMA/ionomycin in PBS, and then how much to add to my cells.
Working...
X